Journal: Scientific Reports

Article Title: Exogenous H 2 S reduces oxidative stress induced by lipid mixture in HepG2 cells through USP22/SIRT1 axis

doi: 10.1038/s41598-025-04924-2

Figure Lengend Snippet: GYY4137 elevated the expression of antioxidant related proteins in LM group cells. ( A ) Western blot analysis of CAT, with α-Tubulin used as a loading control. ( B ) Western blot analysis of SOD, and α-Tubulin used as a loading control. ( C ) Western blot analysis of GSH, with α-Tubulin used as a loading control. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01. LM, lipid mixture; GYY (200), GYY4137 (200 µM).

Article Snippet: Rabbit anti-α-Tubulin primary antibody (Cat#11224-1-AP, RRID: AB_2210206), rabbit anti-SIRT1 primary antibody (Cat#13161-1-AP, RRID: AB_10646436), rabbit anti-CAT primary antibody (Cat# 21260-1-AP, RRID: AB_10733099), rabbit anti-SOD primary antibody (Cat# 10269-1-AP, RRID: AB_2193750) and rabbit anti-GSH primary antibody (Cat#15712-1-AP, RRID: AB_2878171) were purchased from Proteintech (Wuhan, China).

Techniques: Expressing, Western Blot, Control

Journal: Scientific Reports

Article Title: Exogenous H 2 S reduces oxidative stress induced by lipid mixture in HepG2 cells through USP22/SIRT1 axis

doi: 10.1038/s41598-025-04924-2

Figure Lengend Snippet: GYY4137 depended on USP22 to regulate SIRT1 to alleviate LM induced oxidative damage in HepG2 cells (A) Lipid droplets were visualized by ORO staining (25 μm). (B) The levels of MDA and SOD. (C) The TNF-α and IL-6 levels. (D) Detection of ROS content by flow cytometry. (E) Western blot analysis of CAT, SOD and GSH, with α-Tubulin used as a loading control. Data were presented as mean ± SEM. # P < 0.05, ** P < 0.01, ns: no statistical difference. LM, lipid mixture; GYY (200), GYY4137 (200 µM); NC, negative control; siRNA, small interfering RNA.

Article Snippet: Rabbit anti-α-Tubulin primary antibody (Cat#11224-1-AP, RRID: AB_2210206), rabbit anti-SIRT1 primary antibody (Cat#13161-1-AP, RRID: AB_10646436), rabbit anti-CAT primary antibody (Cat# 21260-1-AP, RRID: AB_10733099), rabbit anti-SOD primary antibody (Cat# 10269-1-AP, RRID: AB_2193750) and rabbit anti-GSH primary antibody (Cat#15712-1-AP, RRID: AB_2878171) were purchased from Proteintech (Wuhan, China).

Techniques: Staining, Flow Cytometry, Western Blot, Control, Negative Control, Small Interfering RNA

Journal: Biology

Article Title: Neuroprotective, Antioxidant and Anti-Inflammatory Effect of Greek Pomegranate Seed Oil on N2a Neuroblastoma Cells and Mild Cognitive Impairment Patients.

doi: 10.3390/biology14050548

Figure Lengend Snippet: Figure 4. (a) Representative Western blot images showed the expression levels of superoxide dismu- tase 1 (SOD1) across different experimental conditions (see Supplementary Material Section S1.1). (b) Quantification of SOD1 protein levels normalized to β-actin. (c) Measurement of malondialde- hyde (MDA) levels under oxidative stress conditions induced by 100 µM H2O2. Untreated and H2O2-treated groups were analyzed in the presence of different PSO concentrations (0, 0.2, 10, and 25 µg/mL). Statistical significance: * p < 0.05; *** p < 0.001, with asterisk (*) indicating the comparison versus control group; ## p < 0.01, ### p < 0.001, #### p < 0.0001, with (#) indicating the comparison versus H2O2; $ p < 0.05, with ($) indicating the comparison versus 0 µg/mL PSO. Data are presented as the mean ± SD.

Article Snippet: For the detection of APP, Aβ42, tau, p-tau181, iNOS, IL-1β, TNF-α and SOD1, the following primary antibodies were employed: anti-APP mouse monoclonal antibody (#60342-1-Ig, Proteintech, Manchester, UK), anti-Aβ42 rabbit monoclonal antibody (#14974, Cell Signaling Technology, Danvers, MA, USA), anti-rabbit tau monoclonal antibody (#46687, Cell Signaling Technology, Danvers, MA, USA), anti-ptau181 rabbit monoclonal antibody (#12885, Cell Signaling Technology, Danvers, MA, USA), anti-iNOS rabbit polyclonal antibody (#18985-1-AP, Proteintech, Manchester, UK), anti-IL1β rabbit monoclonal antibody (#L0328Y, Cusabio, Houston, TX, USA), anti-TNF-α mouse monoclonal antibody (#sc-52746, Santa Cruz Biotechnology, Heidelberg, Germany), and an anti-SOD1 rabbit polyclonal antibody (#10269-1-AP, Proteintech, Manchester, UK).

Techniques: Western Blot, Expressing, Comparison, Control